Gene and protein expressions of both p53 and bcl2 were significantly altered in response to extracts. Antitumor activity of extracts of caulerpa taxifolia in. This extract concentration was nontoxic to vero cells and also caused more than 50% cytotoxicity to hep2 cells. The activity of ci in the treated cells was increased compared to cells not treated with our polyphenolic extracts, and a similar trend was detectable for. Evaluation of the cytotoxic activity of extracts from. Our results indicated that inhibition of emt or inhibiting the function of emttfs may be another mechanism involved in the antitumor activity of these herbal plant extracts. All three plant extracts exhibited dose and timedependent killing capabilities in various human derived tumor cell lines and primary cultures established from patients biopsies. Study on antioxidant and antitumor activities of some. Forty four extracts from sixteen plants used traditionally as anticancer agents were evaluated in vitro for their antiproliferative activity against. Antimicrobial activity of medicinal plant extracts and their. The plant has also been used in india and pakistan as a cardio tonic, digestive aid and to improve respiratory function in cases of bronchoconstriction23.
In vitro anticancer activity and cytotoxicity screening of. In vitro antioxidant and anticancer activity of leea indica leaf extracts. These drugs were first discovered during an investigation of the plant as a source of potential oral hypoglycemic. The method of nakahara and co workers 2, 3 was used as an assay for antitumor activity. Study on antioxidant and antitumor activities of some herbal. Antimicrobial activity of medicinal plant extracts and their synergistic effect on some selected pathogens desalegn amenu college of natural and computational science, biology department, wollega university, p. Antitumor activity of aqueous extracts of edible mushrooms. An exploratory study was designed to evaluate the in vivo antitumor activity of cleistanthin b using different mouse tumor models. Brazil, were subjected to screening for antitumor activity against the tumor cell lines, hl60 myeloid leukemia. Modern phytomedicine edited by iqbal ahmad, farrukh aqil, and mohammad owais. Antitumor activity of the plant extract morin in tongue. Accumulating evidences 85,86,87,88 demonstrate that tumor growth and lethality are dependent on angiogenesis. Pdf anticancer activity of morus nigra leaves extract researchgate. Pdf plants are screened for treatment of many ailments including cancer because they possess certain potential constituents which are effective for.
Original articles in vitro antitumor activity of extracts. Box, 395, ethiopia abstract antibiotics provide the main basis for the therapy of microbial bacterial and fungal infections. Don was assayed using potato disc bioassay through agrobacterium tumefaciens infection. The cytotoxic activity of the extracts of cocculus hirsutus on mcf7 cells was investigated in vitro through mtt assay. In the present study antitumor activity of catharanthus roseus was evaluated using both in vitro and in vivo methods. In our study, we have noticed that the cytotoxic activity of a. Helianthae 5 and anthemideae 11, 228 different extracts were prepared from selected plant organs. The antioxidative activity of a total of 92 phenolic extracts from edible and nonedible plant materials berries, fruits, vegetables, herbs, cereals, tree materials, plant sprouts, and seeds was examined by autoxidation of methyl linoleate. Nov, 2014 supplementary material describes the screen of 17 plants that were initially selected and tested for anticancer activity based on their historical and traditional use in treating cancer. Based on the fact that herbal medicine is a strong.
Sevendayold sarcoma 180 ascites tumor cells were implanted. Biological screening of various medicinal plant extracts for. Biological screening of various medicinal plant extracts. Preparations of the plant are commonly used across nigeria for the treatment of inflammatory conditions. With regard to antitumor activity, the mtt assay for acetone extract of stevia rebaudiana treated cells showed that 1. In conclusion, antibacterial and antitumor activities of 51 different extracts obtained from 16 different plants grown in turkey were evaluated. Good antitumor activity was observed with all extracts of a.
Many studies reported that plant derived extracts containing antioxidant principles showed cytotoxicity towards tumor cells and antitumor activity in experimental animals. Evaluation of in vivo antitumor activity of cleistanthin b. Some plant extracts exhibited antiproliferative activity toward all the cancer cell lines. Invitro and invivo antitumor activity of catharanthus roseus. Pdf antitumor, cytotoxic and antioxidant potential of. A multitude of new species are likely to be discovered from miscellaneous habitats, such as soils and tropical forest plants, associated to insects and in the aquatic. Dal cell line induced solid tumor mouse model was used for study iii. Evaluation of antibacterial and antitumor activities of some. Taken together, we first demonstrated the antitumor activity of ferulic acid in vitro and in vivo, and then we confirmed that ferulic acid could suppress breast cancer. High antitumor activity of ethanolic extracts of papuas. Antiviral and antitumor activity of licorice root extracts. Traditional herbal practices, in contrast, involve the use of whole plants or crude extracts of whole. The mtt assay results were used to evaluate the anticancerous activity of the extract.
Microbial transformation can strengthen the antioxidant and antitumor activities of polyphenols. Morin is a naturally occurring bioflavonoid originally isolated from members of the moraceae family of flowering plants and it possesses antitumor activity in various human cancer cells. This first selection resulted in 25 extracts that exerted. Newman encyclopedia of life support systems eolss catharanthus roseus g. Increasingly, natural products have potential for the treatment of cancer. Supplementary material describes the screen of 17 plants that were initially selected and tested for anticancer activity based on their historical and traditional use in treating cancer. Also an increase in caspase 3 activation was seen after extract treatment which is a key stage in apoptotic cell death 36. Antitumor activity of plant cannabinoids with emphasis on. Results the antibacterial activities of the solvent extracts of stevia rebaudiana showed significant variations as shown in table 1. Antitumor and antiviral activity of colombian medicinal plant extracts. Antitumor screening of crude extracts of ten medicinal plants of. Only anecdotal publications have been devoted to the anticancer activ. Biological screening of various medicinal plant extracts for antibacterial and antitumor activities article pdf available in turkish journal of biology 366. Antimicrobial activity of medicinal plant extracts and.
Aug 16, 2006 flavonoidrich extracts from the mature roots of scutellaria baicalensis have been shown to exhibit antiproliferative effects on various cancer cell lines. Screening of in vitro antioxidant activity of methanolic. Study i and ii were carried out with eac and dal cell lines induced malignant ascites mouse models, respectively. Invitro antimicrobial and antitumor activities of stevia. Screening for antitumor activity of various plant extracts on hela and c 4i cell lines gul ozcan 1, ozlem dagdeviren ozsoylemez2, gizem akman1, walid khalilia2, beliz tezel yetiz2, ali karagoz3, gulay melikoglu4, sezin anil4, sukran kultur5, nurhayat sutlupinar4. New drugs from plant secondary metabolites are being developed and studied for their antibacterial and anticancer activities 5. In vitro and in vivo anticancer activity of root extracts of. Strong antibacterial activity was obtained with ethanolic extract of p.
Antioxidant activity of the plant extracts were characterized by using dpph free radical scavenging method. The antitumor effect of a single injection of the test peptides was evaluated by the delay of the appearance and growth of palpable breast cancer in mice over 4 weeks. Thus, we have made an attempt to use this herbal plant to check the efficacy against prostate cancer cell lines. Control wells containing neat solvents negative control were also run parallel in the same plate. Screening for antitumor activity of various plant extracts. Among these plants, cumbens is more potent antioxidant activity then others. The transplanted animals with sarcoma 180 n 24, were divided into four groups n 6 treated with two extracts. Pdf antitumor activity of leaf extracts of catharanthus.
Anticancer activity of extracts derived from the mature roots. In vivo anticancer activity of basella alba leaf and seed extracts. Aims of the study were to examine the high antitumor activity of ethanolic extracts of papuas ant nest plant on an oral carcinoma cells through the growth inhibition and apoptosis induction of kb cells. About 50g of coarsely powdered plant materials 50g250ml were extracted in a soxhlet extractor for 8 to 10 hours, sequentially with petroleum ether, chloroform, ethyl acetate, methanol and water. To identify pure compounds responsible for the detected cytotoxic activity of the extracts of tamus communis l.
A total of 1 ml of plant extracts were diluted with 200 l of distilled water followed by the addition. Both extracts caused a significant reduction of cell viability in the breast and bone cancer cells in a concentration dependent manner. The killing activity was specific toward tumor cells, as the plant extracts had no effect on primary cultures of healthy human cells. We investigated the antitumor activities of other plant cannabinoids, i. Antitumor activity of methanol leaf extracts of catharanthus roseus l. Polyphenols contents, antioxidant and antitumor activities of pine polyphenols and its biotransformation extracts by aspergillus niger, aspergillus oryzae, aspergillus carbonarius, aspergillus candidus, trichodermas viride, mucor wutungkiao and rhizopus sp. In vitro screening for cytotoxic activity of herbal extracts. Ferulic acid exerts antitumor activity and inhibits. Sfe from bidens pilosa linne to obtain extracts rich in. The polyphenol plant extracts have previously been demonstrated to act as chemopreventive and anticancer agents. Potent antitumor property of allium bakhtiaricum extracts. Antioxidant, anticancer activity and phytochemical. The present study explored the antitumor effects of morin in tongue squamous cell carcinoma tscc cells in vitro and investigated the underlying molecular events. The effects of plant leaves extract on cancer cells was studied.
In vitro antibacterial and antitumor activities of some. The extracts contained a very wide range of chemical compounds including borneol, caffeic acid, carrageenan polysaccharide, cineole, cinnamon oil, citral, curcumin and the effects of these against hsv2 were investigated. College of pharmacy and vocational studies, muzafffarnagar, uttar pradesh, india. Effect of whole plant extracts 5, 10, and 11 is timedependent and specific. Anticancer activity of carica papaya extracts in and. Therefore it is very important to study endemicnonendemic plant extracts with. The aim of the present study was to assess antioxidant, antibacterial and antitumor activity of ethanolic extract of the psidium guajava leaves. Apocynaceae, which was used by various cultures for the treatment of diabetes. However, some plants have not yet been scientifically proven to exert anti tumor activity. Antioxidant, antibacterial and antitumor activity of. Anticancer potential of plants and natural products. Concentrationresponse curves for the antiproliferative. Extracts obtained by hydroethanol maceration hce and supercritical fluid extraction sfe were monitored for antitumor activity and the presence of polyacetylenes in the constitution. In vivo antitumor potential of extracts from different.
In vivo antitumor potential of extracts from different parts. Since all these plants were showed some anticancer. Antitumor activity of extract and isolated compounds from drechslera. Pdf antitumor, cytotoxic and antioxidant potential of aster. Comparison in antioxidant and antitumor activities of pine. Pdf evaluation of some plant extracts for standardization and. To demonstrate the potential biological activity of alcoholic extracts, we carried out a screening of the extracts by measuring their in vitro antiproliferative properties against several common human cancer cell lines. We assessed the ability of an ethanolic extract of s. Evaluation of antitumor activity of peptide extracts from. Ic 50 values of total extract and different fractions of a. Antitumor evaluation of two selected pakistani plant extracts. Screening for antitumor activity of various plant extracts on.
To investigate previously isolated, but not tested. Reagents tannic acid, quercetin, lapachol, 1,1diphenyl2picrylhydrazyl dpph, butylhydroxyanisole bha, folin. The plant possesses antiinflammatory activity on carrageenaninduced rat paw edema and it has shown 82% edema inhibition when compared to indomethacin. Our hypothesis was that whole plant extracts selected according to ethnobotanical sources of historical use might contain multiple molecules with antitumor activities that could be very effective in killing human cancer cells. It contains polyacetylenes which may be the constituents responsible for its antitumor activity. All the extracts caused loss of cell viability in a concentration and time dependent manner on the cell lines. The results of aqf showed maximum brine shrimp cytotoxic activity with ed50 values of 154.
Pdf biological screening of various medicinal plant. Antioxidant activity of plant extracts containing phenolic. Anticancer activity of methanol extract of ficus carica leaves and. Extracts of nine species of plants traditionally used in colombia for the treatment of a variety of diseases were tested in vitro for their potential antitumor cytotoxicity and antiherpetic activity.
Oct 27, 2016 the activity of ci in the treated cells was increased compared to cells not treated with our polyphenolic extracts, and a similar trend was detectable for the cv atphydrolase activity in the. In this study, we tried to demonstrate the antitumor effect of these plant extracts on hela and c 41 cells. The minimum concentration of plant extract that was nontoxic to vero cells but toxic to hep2 cells was recorded as the effective drug concentration. Aims of the study were to examine the high antitumor activity of ethanolic extracts of papuas ant nest plant on an oral carcinoma cells through the growth inhibition and apoptosis induction of. The constituents were later approved as vinblastine, used to treat hodgkins disease, and vincristine, used to treat acute childhood leukemia 826. Inhibition of tumor growth, increase in survival time of animal with treatment. The processed plant was dried in the shade at room temperature. Antitumor and antiviral activity of colombian medicinal. Pdf antitumor and antiviral activity of colombian medicinal. Isolation, structural elucidation of flavonoid constituents from leptadenia pyrotechnica and evaluation of their toxicity and antitumor activity. Sep 26, 2008 we studied antitumor effects of peptide extracts from plants on slowly growing mammary adenocarcinoma in cbrbrb8. The nature of cytotoxic activity is found to be apoptotic rather than necrosis by hoechst test. Antioxidant and in vitro cytotoxic activity of extracts of. Herbal products are worldwide accepted as a source of complementary and alternative medicine in 14 various diseases especially in cancer 15.
These extracts were initially screened on three human cancer cell lines at 10 gml. High antitumor activity of ethanolic extracts of papuas ant. Plant extracts 5 and 11 were added at two different concentrations 1. Preparation of plant extracts fresh plant material was washed under running tap water, air dried and powdered. Cytotoxic activity of allium bakhtiaricum extracts. Evaluation of antibacterial and antitumor activities of. Cell lines derived from primary and recurrent brain tumors from the same patient and cells selected for. On the other hand, water extract, especially the flavonoidrich fraction, had higher antihsv activity than did the alkaline extract. The plant extracts induced apoptosis which was demonstrated by an increased subg 1 phase population of cells with lower dna content and condensation of chromatin. Petroleum ether 4060 c, chloroform, ethyl acetate and methanol 80% extracts of c. Today, the use of natural products to treat cancer are very common, particularly in underdeveloped or developing countries. The results showed antioxidant activity using dpph were found to be increased in a concentration dependent. Similarly, in our study, strong antitumor activity was observed with all tested extracts of p.
Peptides from hypericum perforatum and a mixture of. In the framework of our chemical and biological investigations on plant species, the methanol and chloroform extracts from wood and leaves of the six thai. Other plant extracts, chemical compounds, and products have been studied for activity against hsv2 both in vitro and in vivo. Anticancer activity and phytochemical composition of wild gundelia. Antiproliferative activity of plant extracts used against. This screen was the basis for selecting the most effective plant extracts that were further studied in details for their anticancer activity. Ethanol extracts were evaluated for cytoxicity by using mtt assay on llcmk2 cells and by brine shrimp lethality assay. In vitro antitumor activity of different extracts methanol, ethanol, chloroform, ethyl acetate and acetone of catharanthus roseus was evaluated by the mtt assay method using mcf breast cancer cell lines. Evaluating medicinal plants for anticancer activity. Extracts from a broad spectrum of plant species contain substances that possess antitumor activity 1. Vitex negundo, methanol leaf extract, potato disc bioassay, antitumor activity, antibacterial activity introduction bioassay offers special advantages in establishing the biological activities antitumor, antimicrobial, cytotoxic and phytotoxic etc.
The antioxidant activities were observed all three plant extracts and the ec50 values of g procumbens a. Antitumor activity of plant cannabinoids with emphasis on the. Evaluation of both nhf and aqf fractions for sulforhodamine b assay on human cell line ht144 showed ic50 values of 1. Anticancer activity of aloe vera and calligonum comosum. Alkaline extract of licorice root had higher antihiv activity than did water extracts, confirming our previous reports. Antitumor and antiviral activity of colombian medicinal plant extracts article pdf available in memorias do instituto oswaldo cruz 944.
120 1107 1219 502 1475 1186 907 162 501 86 372 105 1416 942 175 213 1567 682 1275 1316 1201 1261 192 964 353 1324 649 951